Preliminary observations indicate that vascular endothelial cadherin (VE cadherin), a major component of endothelial adherens junctions, is important in EC barrier dysfunction. Ischemic and inflammatory diseases target the vascular endothelium and are characterized by cytokine production and endothelial (EC) barrier dysfunction. This technology is capable of detecting differences in protein expression in microdissected ovarian cancer tissue. The project focuses on the use of surface enhanced laser desorption and ionization in the discovery of new biomarkers for ovarian cancer. Specific aims are to determine the signaling pathways in ONOO-mediated enterocyte apoptosis, and to examine the mechanisms by which NO or ONOO inhibits tissue repair mechanisms in enterocytes. The goal of this proposal is to determine the mechanisms by which overexpression of nitric oxide (NO) or its reactive nitrogen species, ONOO, may promote tissue injury (enterocyte apoptosis) and inhibit tissue repair mechanisms (epithelial restitution via enterocyte migration and proliferation), thereby leading to gut barrier failure.
The sample executive summaries are in the three project areas of basic/biomedical research, clinical investigation, and health services research/epidemiology.
